Fig. 4

Or22a is transportable through a Grasp65-dependent unconventional route of protein secretion. A The Golgi reassembly and stacking protein 65 (Grasp65) is a marker of unconventional secretion. Somatic Or22a puncta shown here is colocalized with Grasp65 (blue arrowheads). B Magnification of an Or22a OSN bearing enlarged Or22a puncta. White dashed lines indicate an Or22a punctate. Blue dashed lines indicate the somatic body. Grasp65 as well as Or22a concentrate with a bias around the presumed punctate membrane. C, D Grasp65 knockdown largely abrogates soma Or22a puncta. Magnification of an Or22a OSN in a Grasp65-IR antenna reveals a void in the space where the soma puncta typically manifests. E, F Quantitative analyses for Or22a puncta formation and comparative cilia to soma Or22a intensity in Grasp65-IR flies. For controls, n = 23; for Grasp65-IR, n = 15. For all, error bars represent mean SEM. Two-tailed Student’s t-test was applied for statistical analyses