Fig. 5

Starvation, not sugar overconsumption, encourages the unconventional transport of Or22a. A Or22a staining in w¹¹¹⁸ flies in comparison to those deprived of food for 24 h. B A general increase in Or22a puncta frequencies were observed in starved flies. C Soma Or22a intensity levels are lower in starved than in fed flies, an implication that Or22a puncta are functional vesicles for its transport into the cilia. For both B and C, fed n = 14; starved n = 10. A high-sugar diet, known to alter peripheral neuron activity, had no immediate effects on Or22a transport. E, F Quantitative analyses of puncta frequency and cilia to soma intensity in flies fed a control or high-sugar diet. For 6% sugar, n = 10; for 34% sugar, n = 12. For all, error bars represent mean SEM. Two-tailed Student’s t-test was applied for statistical analyses