Fig. 1

Binding of BbOsrR1, 2, and 3 to BbOhmm promoter elements and BbOhmm expression in the wild type and mutant strains. A EMSA analysis of BbOsrR1, 2, and 3 binding to BbOhmm promotor regions. Wild type and three scanning mutant elements (Mutations A, B, and C) were constructed as detailed in the “Methods” section. (B-C) ChIP-qPCR analysis of BbOsrR1, 2, and 3 binding to the BbOhmm promotor region. Fungal cells were grown under no-stress and H₂O₂ (4 mM) or menadione (MND, 60 μM)-stress conditions over the indicated time course. D,E RT-qPCR analysis of BbOhmm expression in the wild type and ΔBbOsrR1, 2, and 3 mutant strains grown under no-stress and H₂O₂ (4 mM) or menadione (MND, 60 μM)-stress conditions over the indicated time course. Standard deviations (± SD) in B–E derived from triplicate experiments. Columns with the same lowercase letters above them are not significantly different (LSD test, P < 0.05)