Fig. 3

Survival of Galleria mellonella larvae with the median lethal time (LT₅₀) following topical application by spray of 1 mL conidial suspension (1 × 10⁷ spores/mL) (A) and injection into the second proleg with 2 μL of 5 × 10⁶ conidia/mL (B). All controls were treated with water. The experiments were repeated twice at different time. The survival data were plotted as Kaplan–Meyer curves and difference of the gene disruption mutant strains or reverse complementation strains from the wild type strain was analyzed using a log-rank test. LT₅₀ was calculated using SPSS 17.0 program. The asterisks (*) and (**) in the column charts denote P < 0.05 and P < 0.01 for the indicated fungal strains versus the WT (t-test), respectively