Fig. 4

ChIP-seq and RNA-seq analysis identify BbOsrR1 target genes. A Identification of BbOsrR1 (gene promoter) targets by comparison of RNA-seq and ChIP-seq under the normal and H₂O₂ (4 mM)-stressed conditions for 30 min. B MEME analysis indicating the likely binding sequence of BbOsrR1. C Partial annotation of identified BbOsrR1 gene targets and their expression patterns. D EMSA verification of three identified gene targets of BbOsrR1: CatA, BbOsrR2, and BbClp1 with indicated BbOsrR1 protein concentrations. The promoter region of each target harboring mutated binding motif was used as the scrambled DNA sequence control (Mutated promoter) as detailed in the “Methods” section