Fig. 6

ChIP-seq and RNA-seq analysis identify BbOsrR2 target genes. A Identification of BbOsrR2 (gene promoter) targets by comparison of RNA-seq and ChIP-seq under the normal, H₂O₂ (4 mM), and menadione (MND, 60 μM)-stressed conditions for 30 min. B MEME analysis indicating the likely binding sequence of BbOsrR2. C Partial annotation of identified BbOsrR2 gene targets and their expression patterns. D EMSA verification of three BbOsrR2 targets: Msg5, a hypothetical protein gene (BBA_06338) and Mcm1 with indicated BbOsrR2 protein concentrations. The promoter region of each target harboring mutated binding motif was used as the scrambled DNA sequence control (Mutated promoter) as detailed in the “Methods” section. E Model of BbOsrR2 regulation of the Fus3-MAP kinase pathway via (negative) targeting of Msg5 and Mcm1, affecting expression of the Ste4, Fus3, and Cdc28 genes. Upregulated genes in the ΔBbOsrR2 mutant are shadowed in red