Fig. 7

ChIP-seq and RNA-seq analysis identify BbOsrR3 target genes. A Identification of BbOsrR3 (gene promoter) targets by comparison of RNA-seq and ChIP-seq under the normal, H₂O₂ (4 mM), and menadione (MND, 60 μM)-stressed conditions for 30 min. B MEME analysis indicating the likely binding sequence of BbOsrR3. C Partial annotation of identified BbOsrR3 gene targets and their expression patterns. D EMSA verification of two identified BbOsrR3 targets: Fus3 and a hypothetical protein gene (BBA_06338) with indicated BbOsrR3 protein concentrations. The promoter region of each target harboring mutated binding motif was used as the scrambled DNA sequence control (Mutated promoter) as detailed in the “Methods” section. E Model of BbOsrR3 regulation of the Fus3-MAP kinase pathway via (negatively) targeting Fus3 and affecting expression of the Ste2, Sst2, and Msg5 genes. Upregulated genes in the ΔBbOsrR3 mutant are shadowed in red