Fig. 5
From: Genomic insights into the seawater adaptation in Cyprinidae
CNE sequence analysis that may be related to osmoregulation. A An 8-bp specific deletion of P. hakonensis and P. brandtii was located near target gene atg7. B A 7-bp specific insertion of P. hakonensis and P. brandtii was located near target gene usp45. C VISTA sequence conservation plot of the P. hakonensis and P. brandtii specific deletion CNE around atg7, using zebrafish as reference. Red arrows indicate missing bp. D, E Luciferase activity assays of the fragments of atg7 (D) and usp45 (E) promoter from Danio rerio, P. brandtii, and P. hakonensis under control (315 mOsmol/kg) or high osmolarity conditions (600 mOsmol/kg) in HEK293 cells. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001