Imbalance of heterologous protein folding and disulfide bond formation rates yields runaway oxidative stress

Table 1 Physiological parameters of recombinant protein secretion strains^a

Strains^b	Μ_max [h^-1]	Y_SX	Y_SE	Y_SG	Y_SA	Y_SCO2	Carbon balance
WN	0.43 +/- 0.014	0.14 +/- 0.001	0.32 +/- 0.041	0.067 +/- 0.009	0.048 +/- 0.0005	0.30 +/- 0.019	0.89
WI	0.40 +/- 0.012	0.13 +/- 0.002	0.35 +/- 0.029	0.055 +/- 0.005	0.056 +/- 0.0047	0.30 +/- 0.014	0.92
WA	0.32 +/- 0.007	0.11 +/- 0.003	0.31 +/- 0.006	0.060 +/- 0.007	0.049 +/- 0.0023	0.30 +/- 0.002	0.84
dN	0.38 +/- 0.005	0.13 +/- 0.004	0.37 +/- 0.025	0.046 +/- 0.003	0.035 +/- 0.0046	0.29 +/- 0.020	0.91
dI	0.29 +/- 0.005	0.08 +/- 0.006	0.32 +/- 0.017	0.081 +/- 0.001	0.046 +/- 0.0011	0.31 +/- 0.007	0.84
dA	0.31 +/- 0.002	0.11 +/- 0.003	0.32 +/- 0.002	0.066 +/- 0.001	0.049 +/- 0.0009	0.30 +/- 0.004	0.85

^aAll yields (Y) are [g/g]. Glucose (S), biomass (X), ethanol (E), glycerol (G), acetate (A), carbon dioxide (CO2).
^bStrain abbreviations as in Figure 1.

ISSN: 1741-7007