Figure 7
CCT130234 and 17- N -allylamino-17-demethoxygeldanamycin (17-AAG) concentration-dependent inhibition of tumor dissemination on matrix protein. (a-c) Day 4 U-87 MG and MDA-MB-231 P (parental variant) spheroids were placed on gelatin-coated plates and treated with the phospholipase C (PLC)γ inhibitor (CCT130234) over a range of concentrations. Controls were treated with vehicle. Images were captured at intervals (from t0 to 72 h) using an inverted microscope and analyzed with Image-Pro Analyzer software. Representative results of three separate experiments are shown for (a) U-87 MG and (b) MDA-MB-231 P. Values are means ± SD; (n = 12). Student t test with Welch's correction was performed relative to 72 h values: (a) vehicle control vs 2.5 to 10 μM P < 0.001; (b) vehicle control vs 2.5 to 10 μM P < 0.001. (c) Representative images for both cell lines at 72 h are shown. Scale bar: 500 μm. (d) U-87 MG, (e) KNS42 and (f) MDA-MB-231 P spheroids were treated with 17-AAG over a range of concentrations and the area of migration at 24 h post treatment quantified. Values are means ± SD; n = 6. Student t test with Welch's correction was performed: (d) vehicle control vs 0.1 to 2.5 μM P < 0.001; (e) vehicle control vs 0.2 μM **P < 0.01, vs 1 and 5 μM P < 0.001; (f) vehicle control vs 0.7 to 2.8 μM P < 0.001. A representative example of three separate experiments is shown for each cell line.