Polyploidization increases meiotic recombination frequency in Arabidopsis: a close look at statistical modeling and data analysis
© Wang and Luo; licensee BioMed Central Ltd. 2012
Received: 25 November 2011
Accepted: 18 April 2012
Published: 18 April 2012
This paper is a response to Pecinka A, Fang W, Rehmsmeier M, Levy AA, Mittelsten Scheid, O: Polyploidization increases meiotic recombination frequency in Arabidopsis. BMC Biology 2011, 9:24.
See research article at http://0-www.biomedcentral.com.brum.beds.ac.uk/1741-7007/9/24
Many species, particularly flowering plants, have usually experienced a state of polyploidy in their evolutionary history. Meiotic recombination creates novel configurations of genetic variants maintained in the genome of a species for facilitating natural and artificial selection. Thus, to understand how a diploid species differs in frequency of meiotic recombination from its polyploid ancestor has a significant impact in both evolutionary biology and plant and animal genetic breeding. It has been well established that the evolution of polyploid genomes is an extremely dynamic process compared to that of diploids, characterized by extensive genetic and epigenetic changes occurring in the nuclear genome following polyploidization [1–3]. Little is known about the mechanism underpinning the genetic changes. To address this fundamental question, Pecinka and colleagues described in a recently published paper by BMC Biology a direct comparison in frequency of meiotic recombination of the diploid and tetraploid genomes of Arabidopsis . One of the most striking methodological challenges to the study is to properly evaluate the recombination parameter in populations of the species at different levels of polyploidy, particularly in autopolyploids. In fact, linkage analysis with autotetraploids has been a historical problem that can be traced back to the pioneering works of the prominent mathematical geneticists [5–7].
There are at least two major challenges to the tetrasomic linkage analysis. Firstly, double reduction, the most distinct characteristic of polysomic inheritance, allows sister chromatids to enter into the same gamete during meiosis and thus cause systematic distortion in allele segregation . Secondly, multiplex allele segregation makes it almost impossible to infer the underlying genotype directly from PRC-based phenotype data even for co-dominant markers such as a single nucleotide polymorphism or simple sequence repeats . To avoid these difficulties, these authors firstly developed a seed-based assay by creating transformants with green and red fluorescent markers expressed under a seed-specific promoter in Arabidopsis thaliana [10, 11]. In parallel, they created diploids, allotetraploids and autotetraploids which carry these fluorescent markers. After carrying out a series of backcross and selection breeding, they were able to create the diploid and tetraploid lines which bear only a single copy of the marker alleles linked on the Arabidopsis chromosome III. These lines were used to create the segregation populations from which marker phenotype data were collected and used for estimation of recombination frequency between the markers .
The method these authors implemented for modeling and analyzing the marker data from diploid and tetraploid (allo- and autotetraploid) populations needs to be formulated on the basis of the disomic and tetrasomic inheritance models. This paper presents statistically appropriate and mathematically rigorous methods for modeling and re-analyzing the datasets.
Notation, model and analysis
We consider segregation of alleles at the two fluorescent marker loci on the Arabidopsis chromosome III in a F2 family from crossing two parental lines at the marker loci. Following notations of Pecinka et al. , parental genotypes at the markers can be denoted by GR/GR and BC/BC for diploid, GR/BC/DE/DE and BC/BC/DE/DE for allotetraploid, and GR/BC/BC/BC and BC/BC/BC/BC for autotetraploid. The parental lines were crossed to generate offspring populations of diploids, allotetraploids and autotetraploids accordingly. Regardless of polyploidy, the offspring populations from mating these parents can be grouped into four phenotypes: yellow (carrying both red and green marker alleles), green (green allele only), red (red allele only) and grey (none of the marker alleles). The number of individuals for each of the four phenotype classes is denoted by n1,n2,n3 and n4 respectively. Let r be recombination frequency between the two markers and α be the coefficient of double reduction at the green marker, which is nearer to the centromere than the red marker locus. The probability of observing each of the four phenotypes in the diploids and allotetraploids depends on only one parameter, r (f i (r), i = 1,...,4), but characterization of the phenotypic distribution in the autotetraploids needs the two parameters, r and α (f i (α,r), i = 1,...,4).
Distribution of seed phenotype and the underlying genotype at the two fluorescence markers in F2 diploid and autotetraploid populations and estimates of the model parameters
Frequency (f i (r))
Obs (n i )
Obs (n i )
Obs (n i )
0.1643 ± 0.0062
0.2770 ± 0.0110
0.0676 ± 0.0121
0.3048 ± 0.0051
Pecinka et al.
0.154 ± 0.009
0.241 ± 0.018
0.205 ± 0.011
In the allotetraploid population where n1= 1484, n2= 275, n3= 298 and n4= 320, the MLE and corresponding standard deviation are calculated as 0.2770 ± 0.0110. The estimate is in contrast to 0.241 with standard deviation = 0.018 in the original report.
results in the MLE of with a standard deviation of 0.0121 (Table 1).
has only one real root, , which is the MLE of the recombination frequency under the tetrasomic model. The estimate has a standard deviation of 0.0051, which was calculated from the second derivative of the likelihood function at the MLE. Based on the estimates of α and r, we can predict the coefficient of double reduction at the red marker from:
which is below the upper bound of the maximum value of double reduction ¼ . The estimates of α and β strongly suggest multivalent pairing among the homologous chromosomes and, in turn, double reduction occurring in the genome region flanked by the fluorescent markers (Table 1).
This article presents likelihood-based methods for estimating the recombination frequency and other relevant parameters from segregating populations of diploids, allotetraploids and autotetraploids. We demonstrate the methods by re-analyzing the datasets published in Pecinka et al. . Re-analysis of the datasets with the methods developed here reveals quantitative and qualitative differences from the original analysis. Firstly, unlike Pecinka et al., the present analysis enables estimation of double reduction at the marker loci under study and discovers significant double reduction at the markers, emphasizing the necessity of taking the tetrasomic nature into consideration in the data analysis. Secondly, the present study differs from the original analysis in the inferred order in frequency of meiotic recombination of allotetraploid and autotetraploid Arabidopsis. Pecinka et al. concluded that meiotic recombination was more frequent in the allotetraploids than in the autotetraploids whilst our results predict differently. Rationale for the present prediction is supported by several aspects. In fact, allotetraploids show strictly disomic inheritance particularly in the present instance where homoeologous chromosome pairing was excluded. In contrast, homologous chromosomes in autotetraploids have a substantially higher chance to pair, which is essential for recombination to occur among the chromosomes . In addition, it has been theoretically demonstrated that autotetraploids have a much higher upper bound value of recombination frequency in autotetraploids (¾) than that in diploids and allotetraploids (1/2) . Thirdly, the estimates obtained from the present analysis are significantly highly supported by the observed experimental data when compared with those from the original analysis in term of likelihood values of these estimates.
Formulation and numerical analysis represented above were programmed in Mathematica  and the programs will be made available from the corresponding author after publication of this paper.
This study was supported by research grants from the Leverhulme Trust (UK) and The National Basic Research Program of China (2012CB316505). LW is supported by the postdoctoral funding of China and ZWL was also funded by the National Natural Science Foundation of China (31071084/C060103).
- Soltis DE, Soltis PS: The dynamic nature of polyploid genome. Proc Nat Acad Sci USA. 1995, 92: 8089-8091. 10.1073/pnas.92.18.8089.PubMedPubMed CentralView ArticleGoogle Scholar
- Song K, Lu P, Tang K, Osborn TC: Rapid genome change in synthetic polyploids of Brassica and its implications for polyploid evolution. Proc Natl Acad Sci USA. 1995, 92: 7719-7723. 10.1073/pnas.92.17.7719.PubMedPubMed CentralView ArticleGoogle Scholar
- Osborn T, Pires J, Birchler J, Auger D, Chen Z: Understanding mechanisms of novel gene expression in polyploids. Trends Genet. 2003, 19: 141-147. 10.1016/S0168-9525(03)00015-5.PubMedView ArticleGoogle Scholar
- Pecinka A, Fang W, Rehmsmeier M, Levy AA, Mittelsten Scheid O: Polyploidization increases meiotic recombination frequency in Arabidopsis. BMC Biology. 2011, 9: 24-10.1186/1741-7007-9-24.PubMedPubMed CentralView ArticleGoogle Scholar
- Haldane JBS: Theoretical genetics of autotetraploids. J Genet. 1930, 22: 359-372. 10.1007/BF02984197.View ArticleGoogle Scholar
- Mather K: Segregation and linkage in autotetraploids. J Genet. 1936, 30: 287-314.View ArticleGoogle Scholar
- Fisher RA: The theory of linkage in polysomic inheritance. Philos Trans R Soc Lond B Biol Sci. 1947, 23: 55-87.View ArticleGoogle Scholar
- Barley NTJ: Introduction to Mathematical Theory of Genetic Linkage. 1961, London: Oxford University PressGoogle Scholar
- Luo ZW, Hackett CA, Bradshaw JE, McNicol JW, Milbourne D: Predicting parental genotypes and gene segregation for tetrasomic inheritance. Theor Appl Genet. 2000, 100: 1067-1073. 10.1007/s001220051388.View ArticleGoogle Scholar
- Melamed-Bessudo C, Yehuda E, Stuitje AR, Levy AA: A new seed-based assay for meiotic recombination in Arabidopsis thaliana. Plant J. 2005, 43: 358-484.View ArticleGoogle Scholar
- Luo ZW, Zhang R, Kearsey MJ: Theoretical basis for genetic linkage analysis in autotetraploid species. Proc Natl Acad Sci USA. 2004, 101: 7040-7045. 10.1073/pnas.0304482101.PubMedPubMed CentralView ArticleGoogle Scholar
- Cormai L: The advantages and disadvantages of being polyploidy. Nat Rev Genet. 2005, 6: 836-646.Google Scholar
- Luo ZW, Zhang Z, Leach L, Zhang RM, Bradshaw JE, Kearsey MJ: Constructing genetic linkage maps under a tetrasomic model. Genetics. 2006, 172: 2635-2635.PubMedPubMed CentralView ArticleGoogle Scholar
- Wolfram S: Mathematica: A System for doing Mathematics by Computer. 2000, Addison-Wesley Publishing Company, Inc. USA, 5Google Scholar
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