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Table 3 Three-way permutational multivariate analysis of variance (PERMANOVA) results comparing parameters between cold-acclimated and warm-acclimated normal thyroid and hypothyroid fish

From: Thyroid hormone actions are temperature-specific and regulate thermal acclimation in zebrafish (Danio rerio)

 

ATa

Hb

TTc

ATa× Hb

ATa× TTc

Hb× TTc

ATa× Hb× TTc

Ucrit

d

       

F

5.319

12.900

14.768

5.711

0.0088

2.079

0.838

d.f

1, 69

1, 69

1, 69

1, 69

1, 69

1, 69

1, 69

P

0.014*

<0.001**

<0.001**

0.017*

0.883

0.121

0.351

RMR

       

F

3.767

1.216

3.069

1.153

0.427

4.479

2.411

d.f

1, 59

1, 59

1, 59

1, 59

1, 59

1, 59

1, 59

P

0.031*

0.273

0.048

0.297

0.653

0.032*

0.103

AMR

       

F

12.790

8.354

12.430

0.567

1.064

0.112

0.200

d.f

1, 59

1, 59

1, 59

1, 59

1, 59

1, 59

1, 59

P

<0.001**

0.004**

<0.001**

0.471

0.305

0.829

0.717

MS

       

F

8.488

9.140

8.251

1.280

0.772

0.352

1.031

d.f

1, 59

1, 59

1, 59

1, 59

1, 59

1, 59

1, 59

P

0.004**

0.004**

0.007**

0.247

0.371

0.637

0.293

LDHe

       

F

11.547

0.642

145.460

5.611

3.886

0.291

2.346

d.f

1, 72

1, 72

1, 72

1, 72

1, 72

1, 72

1, 72

P

<0.001**

0.445

<0.001**

0.018*

0.045*

0.645

0.107

CSe

       

F

0.505

0.010

45.700

2.410

0.040

0.006

0.246

d.f

1, 72

1, 72

1, 72

1, 72

1, 72

1, 72

1, 72

P

0.497

0.991

<0.001**

0.122

0.935

0.992

0.674

COXe

       

F

1.920

2.122

16.849

3.872

1.027

0.361

0.406

d.f

1, 56

1, 56

1, 56

1, 56

1, 56

1, 56

1, 56

P

0.159

0.148

<0.001**

0.044*

0.314

0.551

0.548

  1. Abbreviations: AMR, active metabolic rate, CS, citrate synthase; COX, cytochrome oxidase; MS, metabolic scope; LDH, lactate dehydrogenase; RMR, resting metabolic rate.
  2. aAcclimation temperature.
  3. bThyroid treatment.
  4. cTest temperature.
  5. dSwimming performance.
  6. eMaximal activity.
  7. *P<0.05.
  8. **P<0.01.