Figure 4

Organization of the regenerating RNC during the late post-injury phase (days 6 – 8). (A) Regenerating radial organ complex as viewed from the coelomic side of the body wall. (B – E) Longitudinal sections through the RNC. (B) Low-magnification view of the wound region; hematoxylin and eosin staining. The inset shows a detailed view of the distal tip of the RNC. (C) Double labeling with the glial marker ERG1 (magenta) and the neuronal marker anti-GFSKLYFamide antiserum (green). (C’) and (C”) show these two types of labeling in separate channels. Note the extended zone of glial dedifferentiation and a terminal swelling of the epineural canal (asterisk). (D, E) Double immunolabeling with the ERG1 (magenta) and anti-RS AFRU (green) antibodies. Note that the dedifferentiated radial glial cells at the distal tip of the RNC (E) produce less RS-like material, than do the glial cells in the more proximal regions (D). c, coelom; ce, coelomic epithelium; ec, epineural canal; hc, hyponeural canal; rnc, radial nerve cord; roc, radial organ complex; wg, wound gap; wvc, water-vascular canal.