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Figure 5 | BMC Biology

Figure 5

From: Spatial and temporal in vivo analysis of circulating and sessile immune cells in mosquitoes: hemocyte mitosis following infection

Figure 5

CM-DiI staining suggests hemocytes replicate in circulation. (A) Fluorescence overlay showing small phagocytes (approximately 5 μm diameter), commonly referred to as prohemocytes, alongside typical granulocytes. Hemocytes (Hem) were stained red with CM-DiI, DNA blue with Hoechst 33342, and GFP-E. coli is green. White color signifies overlap of all three fluorescent channels, indicating a high level of phagocytosis. (B-D) Fluorescence overlays showing granulocytes with nuclei that appear to be fused or dividing, suggesting that they are undergoing autonomous cell division. Some of these hemocytes had phagocytosed E. coli.

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