Figure 7

Infection induces mitosis in circulating hemocytes. (A-B) Taxol treatment of hemocytes results in spindle contraction and highly condensed asters. In panel A, tubulin is stained green and DNA is stained blue. In panel B, tubulin is stained red, GFP-E. coli are green, DNA is stained blue, and dotted lines denote the cell boundary. Triple fluorescence samples such as the ones seen in B were used to quantify mitotic indices. (C) Mitotic indices in naïve mosquitoes and infected mosquitoes at different times following immune challenge. Proliferation peaks at nine hours post-infection. Column heights mark the average and whiskers denote the standard error of the mean.