Figure 4
From: Specific NuRD components are required for fin regeneration in zebrafish
Specific Hdac1 inhibition with MGCD0103 impairs fin regeneration. (A) Western blot analysis for acetylation of histone H3 and histone H4 in 4 dpa fin regenerates treated with DMSO or 5 μM MGCD0103 starting from the time of amputation. The level of acetyl-H3 and acetyl-H4 in MGCD0103-treated fins was increased compared with DMSO-treated fins. β-actin and histone H3 were used as loading controls. (B-E) Whole caudal fins at 2 (B), 3 (C), 7 (D) and 10 dpa (E) treated with DMSO or 5 μM MGCD0103 starting from the time of amputation. MGCD0103 treatment for 10 days impaired regenerative growth without affecting the early stages of regeneration. Arrowheads indicate the amputation plane.