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Table 1 Effect of combining the exonuclease and active site mutations in Pol ε gene on mutation rates in haploids in 8C-YUNI101 genetic background

From: Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations

POL2 allele

Mutation rates for different markers*

 

Forward mutation

Reversion

  

Base substitutions

Frameshifts (+1)

 

Canr (× 10-7)

Ura+ × (10-8)

His+ × (10-8)

Mismatch repair proficient strains

wild-type

3.3

1.3

1.1

 

2.5–4.9

1.1–1.7

0.9–3.5

pol2-Y831A

5.1

1.2

1.6

 

3.5–12.1

0.8–2.8

0.5–2.7

pol2-4

27

3.41

452

 

15–42

2.4–5.7

35–78

pol2-4,Y831A

9

1.5

3.93

 

7–15

1.1–1.9

2.3–5.4

Mismatch repair deficient strains (pms1)

wild-type

90

2.8

87

 

72–150

2.4–4.1

54–150

pol2-Y831A

340

124

4705

 

230–420

11–19

270–790

pol2-4

760

1606

16007

 

120–1060

90–420

950–2800

pol2-4,Y831A

270

158

4109

 

190–440

8–55

260–440

  1. *Here and thereafter, median for 9–27 independent cultures with 95% confidence limits below. 114/16 of revertants sequenced were due to C->A transversions 220/20 revertants sequenced contained a +1A frameshift event 316/16 revertants sequenced were due to +1A frameshift 412/14 revertants were due to C->A change 515/15 revertants sequenced were due to +1A frameshift 612/12 revertants sequenced were due to C->A mutation 716/16 revertants sequenced were due to +1A frameshift 812/15 revertants sequenced were due to C->A mutation 914/14 revertants were due to +1A frameshift