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Table 1 Q-PCR analysis of gene expression in SpRunt morphant embryos

From: Evaluation of developmental phenotypes produced by morpholino antisense targeting of a sea urchin Runx gene

Function

Gene

24 hpf

48 hpf

Transcription factor

Runt

NS

NS/NS/NS

 

Myb

-3.9/NS/NS

NS/NS/NS

 

Dri (OE)

-2.3/NS/-2.5

-12/-8.9/-6.9/-2.6/-7.6/-3

 

Hnf6 (OE)

NS/NS

-2.5/NS/-3.1/-2.5

 

Gsc (OE)

-2.4/NS/NS

-3.7/-2.3/-2.4/-4.7/-5.1

 

Otxα (VPEM early)

NS/NS

+5.2/+4.8/+10.5/+10.2

 

Otxβ1 (OE)

-2.4/-1.9

NS/NS/NS/NS

 

Otxβ3 (VPEM+OE)

-2.4/-2.4

NS/+3.5/NS/NS/NS

Cell cycle control

CyclinD

NS/NS/NS

-2.9/-2.7/-4.8/-2

 

CyclinE

NS/-2

NS/-2.6/NS/NS

 

CyclinA

NS

NS/+3.6/+5.8/+3/+2

 

CyclinB

-2.4/-2.5/-2.2

-3.7/NS/NS

 

Wee1

NS/NS

-2.5/NS/NS/NS

 

Cdk4

NS

NS/NS/NS

Signal transduction

PKC

NS/-2/-3

-8.8/-8/-7.7/-4.9/-5.4

AE differentiation

CyIIIa*

-2.7/-1.9/-2

-2.4/-2.3/-3.8/-3

 

Spec2a

-2.1/-2.3/-2.3/-3.4

NS/NS/NS/-3.9

 

ARS

-3.9/-2.4

NS/NS/NS/NS

VPEM differentiation

Endo16

-6.8/-3.4

-1.8/-2.4/NS/NS/NS

SM differentiation

SM50

-2.4/-2.3/-2.6

-9.6/-3/-4.3/NS/NS/NS

 

SM30

NS/NS

-4.1/NS/NS/NS

 

Msp130

-5.7/NS

NS/-2.7/NS/NS

  1. *CyIIIa was identified previously by cis-regulatory analysis as a direct target of SpRunt [8]. Each number indicates fold-difference in transcript levels in SpRunt-m5-injected embryos compared to controls (triplicate average for a single experiment), measured as described in Methods. Based on the results obtained with CyIIIa, fold-differences of less than a factor of 2 (~1 cycle) were scored as not significant (NS). hpf, hours post-fertilization; AE, aboral ectoderm; OE, oral ectoderm; VPEM, vegetal plate endomesoderm (gut and secondary mesoderm); SM, skeletogenic mesoderm.