Figure 1
From: Runx-dependent expression of PKC is critical for cell survival in the sea urchin embryo
Morpholino antisense-mediated knockdown of SpRunt-1 leads to ectopic proliferation and apoptosis in the gastrula stage (48 h) embryo. (A-B) Differential interference contrast (DIC) images of embryos injected with (A) control MASO and (B) SpRunt-1 MASO. (C-D) Confocal micrographs (single optical sections) of BrdU-labeled embryos injected with (C) control MASO and (D) SpRunt-1 MASO. (E-F) Confocal sections of TUNEL (green)- and DAPI (blue, DNA stain)-labeled embryos injected with (E) control MASO and (F) SpRunt-1 MASO. (G-H) Confocal sections of antiactivated caspase-3 (green)- and DAPI (blue)-labeled embryos injected with (G) control MASO and (H) SpRunt-1 MASO. Scale bar = 20 μm.