Figure 4 From: Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization Subcellular localization analysis of hLsg1. (A) Subcellular localization of YFP and CFP tagged hLsg1 – HeLa cells were transiently transfected with hLsg1-YFP (1, 2), or CFP-hLsg1 (3) and visualized by fluorescence microscopy. (B) Localization of endogenous hLsg1. hLsg1 was visualized by staining with anti-hLsg1 antibodies, followed by Alexa 488-conjugated anti-rabbit antibodies. HeLa cells were transiently transfected with YFP-tagged lamin or Clontech ER-YFP marker. Coilin was visualized by monoclonal mouse anti-coilin antibodies, followed by rhodamine-conjugated anti-mouse antibodies. Bars = 10 μmBack to article page