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Figure 4 | BMC Biology

Figure 4

From: Increased synaptic microtubules and altered synapse development in Drosophila sec8 mutants

Figure 4

Fewer synaptic glutamate receptors are electrophysiologically detectable in sec8 mutants. A: Portions of representative patch clamp recordings from control and sec8Δ1 mutant first instar larvae (22–24 h AEL), showing spontaneous excitatory junction currents (sEJCs) from the muscle 6 NMJ. B: Glutamate-gated currents triggered by pressure ejection of 1 mM glutamate on to patch-clamped muscle 6, from control and sec8Δ1 mutants. Amplitudes of glutamate-gated currents are quantified in C. C: Quantification of glutamate-gated current amplitudes from control and sec8Δ1 mutants. D: sEJCs from control and sec8Δ1 mutant first instar larvae, showing delayed single channel closings observable during the falling phase of the currents. Lines have been added to the figure to indicate the baseline and single receptor current amplitude. Single-channel glutamate receptor amplitudes were measured from delayed closings such as these; quantification of these amplitudes is shown in E. E: Quantification of single glutamate receptor current amplitudes from control and sec8Δ1 mutants. F: Portions of representative two-electrode voltage clamp recordings from control and sec8P1 third instar larvae (110–120 h AEL), showing spontaneous excitatory junction currents (sEJCs) in the muscle 6 NMJ. On the right are shown portions of recordings at expanded time scale, which sacrifices an overall impression of sEJC amplitude differences but better demonstrates the resolution with which larval sEJCs were detected. G: Cumulative frequency histogram of sEJC amplitudes in third instar animals. Homozygous sec8P1 mutant animals had fewer large events, compared with controls.

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