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Figure 1 | BMC Biology

Figure 1

From: Long-term starvation and ageing induce AGE-1/PI 3-kinase-dependent translocation of DAF-16/FOXO to the cytoplasm

Figure 1

Resistance to H 2 O 2 requires daf-16 and starvation. L1s with the indicated genotype were exposed to 0. 0.25 mM, 0.5 mM and 1 mM H2O2 for 45 minutes, washed and placed on agar plates containing bacterial food. Images were taken 48 hours later. Further examination revealed that the smaller larvae are alive but permanently arrested at around L2/L3 stage. (A-C) for larvae treated after hatching in liquid medium without food, mutation of daf-16 (B) causes larvae to be much more sensitive than the wild type (A) or daf-16 mutants rescued with the muIs61 transgene (C). (D, E) Larvae treated after hatching on NGM plates with bacterial food are hypersensitive to H2O2. (F.G) Mutations in the AGE-1/AKT pathway affect starvation-induced resistance to H2O2. Quantification of the proportion of permanently arrested larvae with treatments of 0.5 mM H2O2 (F) and 0.25 mM H2O2 (G) for the more sensitive strains (0.25 mM H2O2 had no effect on wild type larvae).

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