Figure 6

Innervation of the oviduct by two types of fibre. Ai. Confocal image from a Wee-P26 fly of nerves immunostained with anti-HRP (magenta) showing nerves running over the surface of the oviduct. Some nerve fibres run longitudinally (L) giving off circumferential branches (c) while others have a more random wandering path (w). Aii. The previous image merged with one of muscle expressing MHC-GFP shows that the circumferential branches run between the circular myofibrils. B. Merged confocal images of the oviduct from the same preparation as Figure 5C. The nerves (magenta) are immunostained as above in a fly expressing CD8-GFP-Shaker (green). Patches of CD8-Shaker fluorescence co-localise (white) with neural boutons (arrows) on the circumferential nerve fibres but are absent from the varicosities on the wandering fibres (w). C. Individual and merged confocal images showing that the expression of dTdc2-GAL4 as reported by UAS-n-syb-spH (green) colocalises (white) with the anti-HRP labelling (magenta) of some nerves but is absent from others. D. Individual and merged confocal images of anti-HRP labelling (magenta) and anti glutamate receptor (GLUR, green). Note that the round patches of GLUR signal are associated with the straight fibres rather than the wandering fibres. Scalebars: All 20 μm.