Figure 6

Expression of reporter constructs in males. (A–C) The int2itr-1 regulatory region drives expression in the gonad of males. Nomarski (A) and fluorescence (B) images of the male gonad region that expresses the int2itr-1::YFP reporter construct (lateral view, anterior left). An overlap of these images is shown in (C). (A–C) are pha-1(e2123ts) young adult males transformed with the extrachromosomal array pha-1+int2itr-1::YFP. The dashed arrow indicates the sperm in the seminal vesicle (SV) and the solid arrow indicates the valve area (V) where the sperm exit into the vas deferens (VD). (D–F) The gpa-1 regulatory region drives expression in spicule neurons. Nomarski (D) and fluorescence (E) images (dorsolateral) of the male tail showing expression of the gpa-1::GFP construct in the two neurons (SPV and SPD, bracket) that innervate the spicules. The top of the spicule is denoted by an arrowhead, with autofluorescence of the spicules posterior to the arrowhead. An overlap of these images is shown in (F). Animal shown is a pha-1(e2123ts) young adult male transformed with the extrachromosomal array pha-1+gpa-1::GFP. (G–I) The unc-119 regulatory region drives expression in ventral cord neurons, but not in the gonad. Nomarski (G) and fluorescence (H) images of the male gonad region and part of the ventral nerve cord that expresses the unc-119::YFP reporter construct (lateral view, anterior left). An overlap of these images is shown in (I). No expression is seen in the gonad. The dashed arrow indicates the sperm in the seminal vesicle, and the solid arrow indicates the area where the sperm exit into the vas deferens. Arrowheads indicate neurons in the nerve cord. Animal shown is a pha-1(e2123ts) young adult male transformed with the extrachromosomal array pha-1+unc-119::YFP.