Initiation of male sperm-transfer behavior in Caenorhabditis elegansrequires input from the ventral nerve cord

Table 1 sy671 phenotype and rescue

Genotype	Initiate/total^a	Rescue^c
Wild type^b	40/40	N/A
unc-18(sy671)	1/39	N/A
unc-18(sy671); Ex[Punc18::unc-18::yfp]	37/42	Yes
unc-18(e81); Ex[Punc18::unc-18::yfp]	27/30	Yes
unc-18(sy671); Ex[Promoterless::unc-18::yfp]	0/26	No
unc-18(sy671); Ex[Pint2itr-::unc-18::yfp (gonad)]	0/84	No
unc-18(sy671); Ex[gpa-1::unc-18::yfp]	3/93	No
unc-18(sy671); Ex[Punc119::unc-18::yfp (neuronal)]	70/106	Yes
unc-18(sy671); Ex[myo-3::unc-18::yfp (muscle)]	0/87	No
unc-18(sy671); Ex[aex-3::unc-18::yfp]	29/34	Yes

^a For all genotypes carrying an extrachromosomal array (Ex), at least three independent lines were assayed except for unc-18(sy671); Ex[Promoterless::unc-18::yfp], for which only two lines each were tested. These results are the combined data from individual lines. See Additional file 4 for results of individual lines.
^b Wild type is strain PS3696, plg-1(e2001); him-5(e1490).
^c Rescue is defined as being different from unc-18(sy671).
(p < 0.001) using ANOVA with a Tukey-Kramer multiple comparisons test (InStat3 software).

ISSN: 1741-7007