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Figure 1 | BMC Biology

Figure 1

From: The roles of calcium signaling and ERK1/2 phosphorylation in a Pax6 +/-mouse model of epithelial wound-healing delay

Figure 1

Morphology and Pax6 expression of cultured corneal epithelial cells. (A) Hematoxylin and eosin staining of confluent Pax6 +/+ and (B) Pax6 +/- corneal epithelial cell cultures. Both in confluence and at the outgrowing edge of new cultures (C, D), the Pax6 +/- cells were morphologically indistinguishable from wild-type. (E) Immunocytochemistry on primary Pax6 +/+ mouse corneal epithelial cells compared with (G) the human corneal epithelial cell line, HCE. (I) Negative control. (F, H, J) DAPI-labeled cells. (K, N) Directly comparable Pax6 immunolabelling of Pax6 +/+ and Pax6 +/- corneal epithelial-cell cultures. (L, N) DAPI labeling. (O) Western blot of protein lysate from Pax6 +/+ and Pax6 +/- corneal epithelial cell cultures probed for Pax6 and β-actin. Scale bars = 100 μm.

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