Figure 5
Activation of purinergic receptors is necessary but not sufficient for wound healing of Pax6 +/- epithelia. (A) Inhibition of purinergic receptors with suramin did not prevent rise of [Ca2+]i in Pax6 +/+ cells directly adjacent to the wound edge 6 seconds after injury (i), but did prevent propagation of the wave to neighboring cells after 16 seconds (ii). (B) No significant difference was detected in the intensities of the suramin-treated group in row 2, but intensity in row 6 was significantly reduced (*, p < 0.001). (C) ATP was added to cells 4 seconds prior to wounding (indicated by arrow). No significant difference in intensity was detected between the curves (p = 0.13, n = 12 and 9 respectively). (D) Suramin inhibited wound healing in Pax6 +/+ cells (*, p < 0.01), but ATP did not improve wound-healing rates in Pax6 +/- cells. Scale bar = 50 μm.