Figure 5

GNA-2 deficiency results in a polarity-osmotic defective (Pod) phenotype. (A) Wild type or gna-2(qa705) stained for DNA (Hoechst stain; blue) and PAR-3 (pink), PGL-1 (pink) or PIE-1 (green), or wild type expressing PAR-2::GFP fed OP50 (control) or gna-2 RNAi and stained for DNA (Hoechst; blue) and GFP (green) (second row). Embryos are at the one-cell stage except wild type stained for PIE-1, which is at the two-cell stage. Wild-type embryos are oriented with anterior (maternal DNA, polar-body end) on the left. gna-2(qa705) or gna-2(RNAi) does not extrude polar bodies therefore it was not possible to determine the maternal end. Images are overlays of Hoechst and antibody staining. (B) DIC images showing osmotic sensitivity of wild type or gna-2(qa705) dissected in water, 150 mM KCl or 300 mM KCl. Arrows point to the eggshell. (C) Plasma-membrane staining of gna-2(qa705) with the lipophilic dye, FM 4–64 (red). In wild type, only the first polar body stains (white arrow). DIC images are shown on the left.