Figure 6

chs-1(RNAi) results in a gna-2(qa705) -like Pod phenotype. (A) DNA (Hoechst) and chitin staining of wild type or chs-1(RNAi). (B) Hatchling number of wild type or chs-1(RNAi) (mean ± SEM, n = 11, x, y: significantly different, p < 0.05). (C) Osmotic sensitivity of chs-1(RNAi) embryos expressing histone H2B::GFP, dissected in water, 150 mM KCl or 300 mM KCl. Images are overlays of histone H2B::GFP (chromatin; green) and DIC. (D) FM 4–64 permeability of chs-1(RNAi). DIC image is on the left. (E) Wild type or chs-1(RNAi) stained for DNA (Hoechst; blue) and PAR-3 (red), PGL-1 (pink) or PIE-1 (green), or wild type expressing PAR-2::GFP fed OP50 (control) or chs-1 RNAi and stained for DNA (Hoechst; blue) and GFP (green) (second row). Images are overlays of Hoechst and antibody staining. (F) DNA (Hoechst) and chitin staining of wild type or mat-2(ax76). (A,E,F) Embryos are at the one-cell stage except wild type stained for PGL-1, which is at the two-cell stage. Wild-type and mat-2(ax76) embryos are oriented with anterior (maternal DNA, polar-body end) on the left. chs-1(RNAi) does not extrude polar bodies; therefore, it was not possible to determine the maternal end.