The eggshell is required for meiotic fidelity, polar-body extrusion and polarization of the C. elegansembryo

Table 1 Summary of meiotic phenotype in gna-2(qa705), chs-1(RNAi) or cej-1+B0280.5(coRNAi) ^a

	*gna-2(qa705)* ^c	*chs-1(RNAi)* ^d	*cej-1+B0280.5(coRNAi)* ^e
1 ^st polar body not extruded, 12 sister chromatids at meiosis II	100	100	76
2 ^nd polar body not extuded	100	100	82
stray chromatin at meiosis I ^f	8	0	6
stray chromatin at meiosis II ^f	15	10	12
SPCC failed to contact cortex ^g	69	30	47
no pseudocleavage furrow	100	100	94

Numbers are % of embryos displaying phenotype
^aembryos examined in utero using a Leica DMLSFA Confocal Microscope.
^bwild type expressing histone H2B::GFP; n = 10
^c gna-2(qa705) expressing histone H2B::GFP; n = 13
^d chs-1(RNAi) expressing histone H2B::GFP; n = 10
^e cej-1+B0280.5(coRNAi) expressing histone H2B::GFP; n = 17
^f3rd, smaller mass of chromatin, separate from 2 major masses of chromatin segregated at anaphase
^gsperm pronucleus failed to come within 1/2 sperm pronucleus diameter of the cortex

ISSN: 1741-7007