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Table 2 The unclassified sequence from the Sargasso Sea is unlikely to represent a fragment of a viral genome. TBLASTN searches were conducted at NCBI using as a query the 59 residues from the Sargasso Sea sequence (Accession AACY01369547) that formed the putative C-extein. These 59 residues are encoded on the complementary strand, from base 556 to base 732. Each search was restricted to one of the six groups outlined below.

From: Multiple, non-allelic, intein-coding sequences in eukaryotic RNA polymerase genes

Accession

Sequences producing significant alignments

E value

Fungi

  

   gb DQ302778.1

Entomophthora muscae AFTOL-ID28, RPB2

4 × 10-9

   gb DQ521419.1

Auricularia auricula-judae AFTOL-ID1681

7 × 10-9

   gb DQ234553.1

Hydnum albomagnum AFTOL-ID 471, RPB2

1 × 10-8

   gb AY485624.1

Hydnum repandum RPB2

1 × 10-8

   gb DQ302787.1

Umbelopsis ramanniana AFTOL-ID 144, RPB2

2 × 10-8

Metazoa

  

   gb XM_793194.1

Strongylocentrotus purpuratus LOC593725

5 × 10-7

   dbj AK114672.1

Ciona intestinalis cDNA, clone:cieg010h22

1 × 10-6

   gb AC007441.9

Drosophila melanogaster clone BACR10E03

2 × 10-6

   gb BT028050.1

Gasterosteus aculeatus clone CNB114-G10

2 × 10-6

   gb U10333.1

Caenorhabditis elegans RNA polymerase II

2 × 10-6

Plantae

  

   emb AJ565937.1

MGU565937 Mimulus guttatus partial RPB2

9 × 10-7

   gb DQ029103.1

Spirogyra sp. UWCC FW670 RPB2

1 × 10-6

   gb AF020844.1

Marchantia polymorpha RPB140 (RPB2)

1 × 10-6

   gb AY596718.1

Tetralocularia pennelii (RPB2)

1 × 10-6

   gb AY563264.1

Selaginella densa RNA polymerase II (RPB2)

1 × 10-6

   emb AJ566358.1

GSP566358 Gardenia sp. Oxelman 2319 (RPB2)

1 × 10-6

Archaea

  

   gb AE010299.1

Methanosarcina acetivorans str. C2A (rpoB)

2 × 10-6

   gb AE008384.1

Methanosarcina mazei strain Goe1 (rpoB)

2 × 10-6

   gb CP000099.1

Methanosarcina barkeri str. fusaro

3 × 10-6

   gb AE000782.1

Archaeoglobus fulgidus DSM 4304 (rpoB1)

4 × 10-6

   emb BX957222.1

Methanococcus maripaludis S2

5 × 10-6

   emb X14818.1

Sulfolobus acidocaldarius rpoB

9 × 10-6

Viruses

  

   gb AF389451.1

Tiger frog virus, complete genome

2 × 10-4

   gb AY548484.1

Frog virus 3, complete genome

4 × 10-4

   gb AY666015.1

Grouper iridovirus, complete genome

0.001

   gb AY150217.1

Ambystoma tigrinum stebbensi virus

0.001

   gb AF397202.1

Regina ranavirus clone PstI-3.8

0.001

   gb AY653733.1

Acanthamoeba polyphaga Mimivirus

0.001

   emb AJ890364.1

Emiliania huxleyi virus 86 isolate Ehv86

0.003

Eubacteria

  
 

No significant similarity found.

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