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Table 1 The effect of RecA and RecA-GFP on genetic recombination.

From: A novel role for RecA under non-stress: promotion of swarming motility in Escherichia coli K-12

Strain

Recombination frequency

Relative recombination

recA + (pCA24N)

2.3 ± 1.1 × 10-2

1.0

ΔrecA (pCA24N)

6.8 ± 2.3 × 10-7

2.9 × 10-5

ΔrecA (pRecA-Htg)

8.5 ± 3.2 × 10-3

0.36

ΔrecA (pRecA-GFP)

7.4 ± 4.5 × 10-6

3.2 × 10-4

  1. Matings were performed as described in Methods. The recombination frequency is the number of transconjugants [CmR, KmR] relative to the number of donor cells. Relative recombination indicates recombination values relative to MG1655 recA+ (pCA24N). Results represent the average of three independent measurements.
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BMC Biology

ISSN: 1741-7007

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