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Table 3 The list of cuticle associated proteins up-regulated in post-moult (Cy3) crabs when compared against crabs in the intermoult stage (Cy5)

From: Gene expression profiling of cuticular proteins across the moult cycle of the crab Portunus pelagicus

Transcript Ids (accession no.)

Gene IDs (BLAST results)

Score (bits)

E value

* M

*t

*P value

*Adjusted P value

PpCUT12 (GenBank: EF102004)

P81580 CUPC1 Cuticle protein CP1158 CPCP1158 Cancer pagurus

70.1

6e-11

4.949

5.485

0.002

0.036

    

4.867

5.669

0.002

0.033

    

4.277

5.495

0.002

0.036

    

4.26

5.313

0.003

0.04

    

4.233

5.532

0.002

0.035

    

4.02

5.129

0.003

0.045

    

3.929

5.259

0.003

0.041

    

3.844

5.262

0.003

0.041

PpBD2 (GenBank: EF102014)

DQ288154 Callinectes sapidus calcified cuticle protein CP15.0 mRNA

745

0.0

4.471

9.086

0

0.007

    

3.709

6.694

0.001

0.019

 

ABB91679 calcified cuticle protein CP15.0 C. sapidus

214

2e-54

3.414

6.097

0.001

0.028

PpCUT13 (GenBank: EF102005)

P81580 CUPC1 Cuticle protein CP1158 (CPCP1158) C. pagurus

69.7

6e-11

4.415

5.963

0.002

0.029

    

4.397

5.489

0.002

0.036

    

4.161

5.83

0.002

0.031

    

2.104

7.01

0.001

0.017

PpCBM (GenBank: EF102017)

AAR06266 peritrophic membrane chitin binding protein 2 Trichoplusia ni (Cabbage lopper) AAD40313 chitinase 1 Penaeus monodon

55.8

6e-07

3.13

5.858

0.002

0.031

  

46.6

3e-04

    

PpVER3 (GenBank: EF102020)

NP_730443 LDLa domain containing chitin binding protein 1 CG8756-PB, isoform B Drosophila melanogaster

110

5e-23

3.024

8.459

0

0.009

PpBD1 (GenBank: EF102013)

DQ288154 C. sapidus calcified cuticle protein CP15.0 mRNA

87.7

3e-14

2.817

4.966

0.004

0.05

    

2.811

5.293

0.003

0.041

 

P81584 CUPC5 Cuticle protein CP1876 CPCP1876 C. pagurus

145

1e-33

2.754

2.754

0.003

0.041

PpBD3 (GenBank: EF102015)

P81584 CUPC5 Cuticle protein CP1876 CPCP1876 C. pagurus

80.1

1e-13

2.687

5.321

0.003

0.04

PpVER1 (GenBank: EF102018)

NP_730442 LDLa domain containing chitin binding protein 1 CG8756-PA Drosophila

155

1e-38

2.609

8.689

0

0.008

PpBD4 (GenBank: EF102016)

P81584 CUPC5 Cuticle protein CP1876 CPCP1876 C. pagurus

53.5

2e-06

1.823

5.729

0.002

0.032

  1. M = log 2 fold change in expression, t = t-statistic, and the P value = t-distribution. The adjusted P values were calculated using the false discovery rate (FDR) procedure. Positive values of M and t indicate up-regulation in the Cy3 sample (* multiple values for the same transcript indicate expression levels for different cDNA probes on the microarray that make up a contig).