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Table 7 The list of cuticle associated proteins down-regulated in crabs in the late pre-moult stage (Cy3) when compared against crabs at ecdysis (Cy5)

From: Gene expression profiling of cuticular proteins across the moult cycle of the crab Portunus pelagicus

Transcript IDs (accession no.)

Gene IDs (BLAST results)

Score (bits)

E value

*M

*t

*P value

*Adjusted P value

PpCUT12 (GenBank: EF102004)

P81580 CUPC1 Cuticle protein CP1158 CPCP1158 C. pagurus

70.1

6e-11

-4.78

-6.756

0.001

0.044

    

-3.931

-6.565

0.002

0.047

    

-3.843

-7.666

0.001

0.03

    

-3.739

-6.428

0.002

0.048

    

-3.46

-6.36

0.002

0.049

    

-3.034

-6.734

0.002

0.044

PpCUT1 (GenBank: EF101993)

P81582 CUPC3 Cuticle protein CP1246 (CPCP1246) C. pagurus

148

3e-34

-4.731

-6.517

0.002

0.048

PpCB1 (GenBank: EF102006)

DQ288153 C. sapidus calcified cuticle protein CP6.1 mRNA

135

1e-28

-4.198

-15.618

0

0.01

 

ABB91678 calcified cuticle protein CP6.1 C. sapidus

83.2

3e-15

    

PpCB3 (GenBank: EF102008)

DQ288153 C. sapidus calcified cuticle protein CP6.1 mRNA

54.0

3e-04

-3.956

-7.798

0.001

0.029

 

ABB91678 calcified cuticle protein CP6.1 C. sapidus

79.7

4e-14

    

PpCUT5 (GenBank: EF102010)

P81580 CUPC1 Cuticle protein CP1158 (CPCP1158) C. pagurus

155

3e-36

-3.674

-6.567

0.002

0.047

PpCUT11 (GenBank: EF102003)

P81580 CUPC1 Cuticle protein CP1158 (CPCP1158) C. pagurus

41.2

0.003

-2.631

-9.379

0

0.018

PpCUT9 (GenBank: EF102001)

P81580 CUPC1 Cuticle protein CP1158 (CPCP1158) C. pagurus

100

5e-20

-2.482

-7.892

0.001

0.028

PpCUT4 (GenBank: EF101996)

P81582 CUPC3 Cuticle protein CP1246 (CPCP1246) C. pagurus

42.0

3e-08

-1.64

-6.397

0.002

0.048

PpVER2 (GenBank: EF102019)

NP_730443 LDLa domain containing chitin binding protein 1 Drosophila

32.0

8.4

-2.676

-9.202

0

0.018

  1. M = log 2 fold change in expression, t = t-statistic, and the P value = t-distribution. The adjusted P values were calculated using the false discovery rate (FDR) procedure. Negative values of M and t indicate down-regulation in the Cy3 sample (* multiple values for the same transcript indicate expression levels for different cDNA probes on the microarray that make up a contig).