Figure 4

Brood production across diet categories over time. Comparison of mean number of pupae produced over time for diets A (artificial diet with essential AA), diet AR (A plus antibiotic), diet B (A minus essential AA) and control (honey water, Bhatkar agar and cockroaches). Numbers of pupae produced are standardized relative values with minimum 0 when no pupae were present in the colony and the maximum number of pupae as 1. A logistic trend analysis revealed that worker groups fed diet AR were significantly slower in the production of pupae compared to groups on the three other diets.