Figure 1

O -fucose is not required during embryonic neurogenesis. Embryos immunostained with anti-ELAV (green) and either anti-HRP or anti-OFUT1 (magenta) antibodies, as indicated. Panels marked prime show separate channels of the same embryo. (A)Wild-type embryo. (B) Ofut1 ^4R6 mutant embryo from Ofut1 ^4R6 germline clone. A neurogenic phenotype is revealed by the expansion of ELAV staining. (C) Ofut1 ^4R6 mutant embryo from Ofut1 ^4R6 germline clone expressing Ofut1 ^R245Afrom a genomic rescue construct(Ofut1 ^R245A [18.1]). OFUT1^R245A expression is visible. ELAV staining shows the absence of a neurogenic phenotype. (D) Gmd ¹ mutant embryo derived from Gmd ¹ germline clone. Absence of fucosylation was confirmed by the absence of the HRP epitope; neurogenesis appears normal.