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Table 3 GenBank accessions and primers used for the cloning of the various plant cDNAs

From: A subgroup of plant aquaporins facilitate the bi-directional diffusion of As(OH)3 and Sb(OH)3across membranes

Name used in this work

GenBank accession number to protein sequence

GenBank accession number to nucleotide sequence

Forward and reverse primer for the cloning of the genes

AtNIP1;1

CAA16760

Y07625

Forward 5' ATGGCGGATATCTCGGGAAAC 3'

Reverse 5' TCAAGTGCTACCGATTCTCACG 3'

Atnip1;1Δ 2–44

  

Forward 5' ATGTCTCTACTCTCAGTCTCTGTCCCT 3'

Reverse 5' TCAAGTGCTACCGATTCTCACG 3'

AtNIP2;1

T02327

AJ276475

Forward 5' ATGGATGACATATCAGTGAGCA 3'

Reverse 5' TCACAGAGGAAGATCGGTAAC 3'

Atnip2;1Δ 2–37

  

Forward 5' ATGTCCCCTCCTTTGCTC 3'

Reverse 5' TCACAGAGGAAGATCGGTAAC 3'

AtNIP5;1

T04053

AY087560

Forward 5' ATGGCTCCACCGGAGGCTGA 3'

Reverse 5' TTAACGACGAAAGCTCCTAACCGG 3'

Atnip5;1Δ 2–67

  

Forward 5' ATGGATTTTCCCTCTCCTGATGTCTC 3'

Reverse 5' TTAACGACGAAAGCTCCTAACCGG 3'

AtNIP6;1

AAF14664

BT020229

Forward 5' ATGGATCATGAGGAAATTC 3'

Reverse 5' TCATCTTCTGAAGCTCCTC 3'

Atnip6;1Δ 2–29

  

Forward 5' ATGGGGAAGAGGAATGGACAC3'

Reverse 5' TCATCTTCTGAAGCTCCTC 3'

Atnip6;1Δ 2–69

  

Forward 5' ATGTCTCTCCCTCCCCCTAA 3'

Reverse 5' TCATCTTCTGAAGCTCCTC 3'

AtNIP7;1

AAF30303

AY087797

Forward 5' ATGAATGGTGAGGCACGGTCA 3'

Reverse 5' TTAACGTAAAAGTGAAGAAACGGAAGG 3'

Atnip7;1Δ 2–35

  

Forward 5' ATGCTCCCCTATGATATAGATCTCAATC 3'

Reverse 5' TTAACGTAAAAGTGAAGAAACGGAAGG 3'

LjNIP5;1

EU294214

ABY19373

Forward 5' ATGCCTCCGCTGTGGAAGAA 3'

Reverse 5' CTAGCGACGGAAGCTCCTAACC 3'

Ljnip5;1Δ 2–59

  

Forward 5' ATGGATTTCTCTGCTGGTATTGG 3'

Reverse 5' CTAGCGACGGAAGCTCCTAACC 3'

LjNIP6;1

EU294215

ABY19374

Forward 5' ATGGACAACAATGAGGACATTCC 3'

Reverse 5' TCACCTTCTGAAGCTGGGGT 3'

Ljnip6;1Δ 2–72

  

Forward 5' ATGTCATTGCCATCCCCTCCT 3'

Reverse 5' TCACCTTCTGAAGCTGGGGT 3'

OsNIP1;1

Q40746

D17443

Forward 5'GGCTTAAUATGGCAGGAGGTGACAACAA3'

Reverse 5'GGTTTAAUTTAGGTGGAGGAGTTCATCCG 3'

OsNIP2;1

Q6Z2T3

AB222272

Forward 5'GGCTTAAUATGGCGAGCAACAACTCCAGA 3'

Reverse 5'GGTTTAAUTCACACTTGAATGTTCTCCATCTCG 3'

OsNIP2;2

Q67WJ8

AP003569

Forward 5'GGCTTAAUATGGCATCGACGACAGCG 3'

Reverse 5'GGTTTAAUTTATACGTTGTCGAACTCGTCGG 3'

OsNIP3;2

Q7EYH7

AP004461

Forward 5'GGCTTAAUATGGAAGGGGGCAAGATG 3'

Reverse 5'GGTTTAAUCTACAGCTTGATTGCAAAATAAAAC3'

At1g02260

AAY56454

BT023463

Forward 5'GGCTTAAUATGGCAATGGCTCCTGTAA 3'

Reverse 5'GGTTTAAUTTACTTGATAAGGAAGAGACCAATC3'

OsLsi2

NP_001048691

NM_001055226

Forward 5'GGCTTAAUATGAGTGAGCTTGCGTCGG 3'

Reverse 5'GGTTTAAUTCAGATCTTGCCGATGAGGG 3'

Os10g0547500

NP_001065297

NM_001071829

Forward 5'GGCTTAAUATGGCACTGGCGTCGCTG 3'

Reverse 5'GGTTTAAUCTAGATGTTGATCTTGCCGATGAGA 3'

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