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Table 1 Activity of tmRNA -1 triplet mutants in the KanR assay

From: The role of upstream sequences in selecting the reading frame on tmRNA

UUU X

UCU X

UAU X

UGU X

UUC 10

UCC 100

UAC 25

UGC 100

UUA X

UCA 35

UAA X

UGA X

UUG X

UCG 100

UAG 100

UGG 50

CUU X

CCU X

CAU 40

CGU X

CUC 50

CCC 50

CAC 100

CGC 60

CUA X

CCA 100

CAA X

CGA X

CUG 100

CCG 100

CAG 90

CGG 20

AUU 90

ACU X

AAU 90

AGU 100

AUC 100

ACC 100

AAC 80

AGC 100

AUA 60

ACA 100

AAA 100

AGA 100

AUG 100

ACG 100

AAG 100

AGG 100

GUU 90

GCU X

GAU 100

GGU 100

GUC 100

GCC 100

GAC 100

GGC 100

GUA X

GCA X

GAA 95

GGA 100

GUG 100

GCG 25

GAG 100

GGG 100

  1. tmRNA directs the addition of the last 15 amino acids of KanR onto ribosomes stalled on a kanR template in Escherichia coli. Only cells with functional tmRNA survive on 30 μg/ml kanamycin [11]. Each -1 triplet mutant was tested individually and the data reported as percentage survival on selective (kanamycin) media. Triplets listed as X showed no survival. Triplets forbidden by the rules developed by Lim and Garber [16] are underlined and in bold type.