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Table 3 Treatment with PUFA does not affect the amounts of PrPC in neuronal membranes

From: Docosahexaenoic and eicosapentaenoic acids increase prion formation in neuronal cells

Treatment

PrPC (ng/ml)

 

Whole cell extract

Lipid raft fraction

Bulk fraction

None

31.6 ± 4.8

25.5 ± 2

2.9 ± 0.9

1 μM AA

30.2 ± 3.1

26.8 ± 2.9

2.8 ± 0.5

1 μM DHA

33.2 ± 4.4

26.6 ± 3.4

3.8 ± 0.8

1 μM EPA

34.5 ± 2.9

27.8 ± 3.1

3.1 ± 0.3

1 μM LA

29.9 ± 4.1

25.9 ± 2.8

3.4 ± 0.8

1 μM LNA

28.8 ± 4.7

24.5 ± 2

3.5 ± 1.2

2 μM Simvastatin

35.6 ± 4.2

7.2 ± 2.2 *

25.9 ± 3.8 *

  1. The amounts of PrPC in whole cell extracts, detergent insoluble membranes (lipid raft fraction) and the detergent soluble membrane (bulk fraction) from primary cortical neurons treated with 1 μM PUFA or 2 μM simvastatin for 24 hours. Amounts of PrPC were measured by ELISA and are expressed as the mean average PrPC (ng/ml) ± SD, n = 12. * = amounts of PrPC significantly different from that of untreated cells (P < 0.01).