Table 1 Primary antibodies used in this study
From: The molecular basis of defective lens development in the Iberian mole
Gene product 1 | Antibody source | Working dilution | References |
|---|---|---|---|
PAX6 | Mouse monoclonal, raised against chicken protein | 1/400 | Developmental Studies Hybridoma Bank, University of Iowa, US |
PROX1 | Rabbit polyclonal, raised against human protein | 1/250 | Sigma (P7124) |
c-MAF | Goat polyclonal, raised against human protein | 1/200 | Santa Cruz (sc-10017) |
FOXE3 | Goat polyclonal, raised against mouse protein | 1/200 | Santa Cruz (sc-48162) |
β-crystallin | Rabbit polyclonal, raised against human protein | 1/100 | Santa Cruz (sc-22745) |
PH3 | Rabbit polyclonal raised against human protein | 1/100 | Upstate (06–570) |