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Figure 5 | BMC Biology

Figure 5

From: Inducible and constitutive heat shock gene expression responds to modification of Hsp70 copy number in Drosophila melanogaster but does not compensate for loss of thermotolerance in Hsp70null flies

Figure 5

Gel electrophoresis of PCR products amplified from individual fly genomic DNAs. In each gel, DNA size standards are in outermost lanes, interior lanes contain products obtained from four individual Hsp70 -, Hsp70 A-Ba-, Hsp70 + and Hsp70 traIIIflies as marked. (A) 1 kb Hsp70 fragment, comprising the 3' half of the Hsp70 CDS. (B) 1 kb fd3F fragment. (C) 140 bp Hsp70 fragment, from the 3' end of the CDS, amplified with a 60°C annealing step; same reaction employed in quantitative PCR. (D) 104 bp RpL32 CDS fragment amplified with a 60°C annealing step; same reaction employed in quantitative PCR. (E) 193 bp Hsp70 fragment, from the 5' end of the CDS, amplified with primers specific to Hsp70Aa/Ab. (F) 193 bp Hsp70 fragment, from the 5' end of the CDS, amplified with primers specific to Hsp70Ba/Bb/Bbb/Bc.

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