Figure 5 From: Inducible and constitutive heat shock gene expression responds to modification of Hsp70 copy number in Drosophila melanogaster but does not compensate for loss of thermotolerance in Hsp70null flies Gel electrophoresis of PCR products amplified from individual fly genomic DNAs. In each gel, DNA size standards are in outermost lanes, interior lanes contain products obtained from four individual Hsp70 -, Hsp70 A-Ba-, Hsp70 + and Hsp70 traIIIflies as marked. (A) 1 kb Hsp70 fragment, comprising the 3' half of the Hsp70 CDS. (B) 1 kb fd3F fragment. (C) 140 bp Hsp70 fragment, from the 3' end of the CDS, amplified with a 60°C annealing step; same reaction employed in quantitative PCR. (D) 104 bp RpL32 CDS fragment amplified with a 60°C annealing step; same reaction employed in quantitative PCR. (E) 193 bp Hsp70 fragment, from the 5' end of the CDS, amplified with primers specific to Hsp70Aa/Ab. (F) 193 bp Hsp70 fragment, from the 5' end of the CDS, amplified with primers specific to Hsp70Ba/Bb/Bbb/Bc.Back to article page