Figure 7
From: An internal ribosome entry site element directs the synthesis of the 80 kDa isoforms of protein 4.1R
The length of the 4.1R sequence inserted in the bicistronic vector determines the expression of the second cistron. A. Schematic representation of the 4.1R sequences introduced into the intercistronic region of the DsRed-EGFP bicistronic vector. Numbers in the intercistronic region (583, 413, 215 and 149) refer to the nucleotides maintained from the 3' region of the 583 nt 4.1R sequence. The fragments were cloned in the sense (s) and antisense (a) orientations. B. The indicated constructs were transfected into COS-7 cells and their fluorescence intensity determined. The EGFP:DsRed ratios are expressed relative to that of the plasmids containing the sequence of 4.1R in the antisense orientations, which were assigned a value of 1. Error bars correspond to the SEM from five experiments.