Figure 4

AUF1 is post-translationally modified in HEK293 cells transfected with CaR. A. 1D gels for AUF1 and α-tubulin as a loading control. B. 2D gel analysis for endogenous AUF1 in extracts from cells transiently transfected with the CaR (CaR+) plasmid or empty vector (CaR-). Molecular weight markers are shown on the right and the four AUF1 isoforms are indicated. C. 2D gel analysis of extracts from cells transiently transfected with the CaR plasmid or empty vector and the myc-AUF1 isoforms p37, p40, p42 or p45 separately. D. 2D analysis of extracts from cells transiently transfected with the CaR plasmid or empty vector and myc-AUF1p40 without and after treatment with a non-specific phosphatase (CIP). The results all represent one of two repeat experiments with similar results.