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Figure 3 | BMC Biology

Figure 3

From: F-actin-based extensions of the head cyst cell adhere to the maturing spermatids to maintain them in a tight bundle and prevent their premature release in Drosophila testis

Figure 3

Actin caps are extended from the head cyst cell. (A) Optical section from the SG18.1Gal4 UAS-GFP/UAS-GFP:actin testes stained with rhodamine isothiocyanate (RITC):phallodin (red) and 4',6-diamidino-2-phenylindole (DAPI) (blue) show GFP and GFP-actin localizations in the head cyst cell nucleus (arrowhead) and in the actin cap (arrows), respectively. (B), (C) A similar staining of the SG18.1Gal4 UAS-GFP:actin/+ testis shows prominent GFP:actin localization in the actin cap (arrows, (B)) and also in the investment cones (C). (D) The actin cap (arrows) is also labeled by GFP:actin in pCOGGal4/Y; UAS-GFP:actin/+ testis. (E)-(G) Confocal sections from the DAPI (blue) stained w pCOGGal4 UAS-myosin VII:GFP/Y testis show myosin-VII:GFP (green) localization around the nuclei bundles (NBs) (arrows, (E)) at the testis base. (F) Higher magnification image of a single NB show prominent myosin VII:GFP localization along the NB (arrowheads) and also in the head cyst cell (arrows). (G) RITC:phalloidin (red) and DAPI (blue) staining further revealed that the myosin VII:GFP is enriched in the actin cap (arrows). The arrowheads indicate an empty actin cap. (See Additional file 3 for a detailed analysis of expression pattern in testes.)

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