Figure 8

Effects of recombinant RBP4 administration on Akt phosphorylation and Glut4 protein expression in Hmga1 -deficient mice. Basal (saline) levels of pAkt (left) and Glut4 (right) were increased in skeletal muscle of saline-injected Hmga1-deficient mice compared with controls, and were reduced following RBP4-injection (n = 6 per genotype). Densitometric quantifications of three independent experiments from 3 animals per genotype are shown, together with representative Western blots of pAkt, Glut4, and serum RBP4 of saline and RBP4-injected mice. *P < 0.05 versus Hmga1 ^+/+.