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Figure 1 | BMC Biology

Figure 1

From: Heterologous cross-seeding mimics cross-species prion conversion in a yeast model

Figure 1

NM PM -C SC but not NM CA -C SC is inactivated in the presence of [ PSI + ]. A. NMPM-CSC and NMCA-CSC provide translation termination function. [PSI +] or sup35 cells ectopically expressing prion domains of either Pichia methanolica (NMPM) or Candida albicans (NMCA) fused to the C domain of Saccharomyces cerevisiae (NMSC) expressed on a URA3 plasmid on media containing low adenine-Ura and -Ade-Ura. -Ura is used to maintain the plasmid. B. NMPM-CSC is inactivated in [PSI +] cells and not in a sup35 mutant. Around 107 [PSI +] cells expressing either NMPM-CSC or NMCA-CSC, or sup35 mutant yeast expressing NMPM-CSC were plated on -Ade-Ura media.

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