Assigning strains to bacterial species via the internet

Table 1 Genes and PCR primers used in viridans group MLSA scheme

Primer	Gene product	Sequence (5'-3')*	Primer length (bp)	Trimmed fragment size (bp)	Annealing temperature (°C)
guaA-up	GMP synthase	ATYCARTTYCACCCMGAAGT	20	567	55
guaA-dn		CWGGNCCWGGRAATGGTTG	19		55
map-up	Methionine aminopeptidase	GCWGACTCWTGTTGGGCWTATGC	23	348	55
map-dn		TTARTAAGTTCYTTCTTCDCCTTG	24		55
pfl-up	Pyruvate formate lyase	AACGTTGCTTACTCTAAACAAACTGG	26	351	55
pfl-dn		ACTTCRTGGAAGACACGTTGWGTC	24		55
ppaC-up	Inorganic pyrophosphatase	GACCAYAATGAATTYCARCAATC	23	552	50
ppaC-dn		TGAGGNACMACTTGTTTSTTACG	23		50
pyk-up	Pyruvate kinase	GCGGTWGAAWTCCGTGGTG	19	492	50
pyk-dn		GCAAGWGCTGGGAAAGGAAT	20		50
rpoB-up	RNA polymerase beta subunit	AARYTIGGMCCTGAAGAAAT	20	516	50
rpoB-dn		TGIARTTTRTCATCAACCATGTG	23		50
sodA-up	Superoxide dismutase	TRCAYCATGAYAARCACCAT	20	378	50
sodA-dn		ARRTARTAMGCRTGYTCCCARACRTC	26		50
tuf-up	Elongation factor Tu	GTTGAAATGGAAATCCGTGACC	22	426	55
tuf-dn		GTTGAAGAATGGAGTGTGACG	21		55

*Sequences are shown using the IUPAC codes for sites where degeneracy was introduced.

ISSN: 1741-7007