Figure 3
tTA and hid Ala5 expression depending on different integration sites. The expression of tTA and hid Ala5 is shown by WMISH performed on embryos from a 0–48 h egg collection of medfly combinations (comb.) carrying both driver (D) sryα 2-tTA and effector (E) TREhs43-hid Ala5 in heterozygous conditions. The developmental stages of embryogenesis are as indicated in Figure 2. The flies were reared on Tc-free adult food. 100% lethality in lab tests is indicated with + and the stage of complete lethality is indicated in brackets.