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Table 2 Average number of interactions (95% confidence intervals (CIs)) per stage and per gonadal or non-gonadal categories in the embryonic and adult stages

From: Ontogeny and phylogeny: molecular signatures of selection, constraint, and temporal pleiotropy in the development of Drosophila

 

No threshold

Greater than twofold

 

n

Mean

95% CI

n

Mean

95% CI

Stage:

      

Embryonic

2,159

8.495

7.884 to 9.107

1,415

8.913074205

8.097 to 9.729

Larval/pupal

858

7.401

6.566 to 8.240

439

6.760820046

5.611 to 7.915

Adult

1,405

6.955

6.291 to 7.619

926

6.457883369

5.713 to 7.201

Stage/tissue (adult gonads combined):

      

Embryonic general

1,504

8.762

8.038 to 9.483

890

9.270

8.219 to 10.339

Embryonic gonad

609

8.612

7.176 to 10.039

331

7.254

6.039 to 8.476

Adult general

682

6.701

5.947 to 7.453

381

6.071

5.134 to 7.018

Adult gonad

973

6.864

6.128 to 7.603

635

6.543

5.639 to 7.453

Stage/tissue (adult gonads separated):

      

Embryonic general

1,280

8.96171875

8.136 to 9.784

721

9.510402219

8.264 to 10.749

Embryonic gonad

520

8.726923077

7.086 to 10.346

263

7.566539924

6.060 to 9.077

Adult general

583

6.491467577

5.698 to 7.278

360

6.088888889

5.104 to 7.073

Adult ovary

713

7.553997195

6.683 to 8.419

334

7.508982036

6.298 to 8.722

Adult testis

763

6.804718218

6.024 to 7.590

539

6.769944341

5.758 to 7.769

  1. Classifications using no threshold and greater than twofold proportional representation threshold are shown. (n) Indicates the number of genes in each category. Larval/pupal represents the pooled larval and pupal stages. The average number of interactions per stage was found to be statistically significantly higher in the embryonic stage as compare to the other two stages (P < 0.5). When the adult stage is separately classified into general, ovaries, and testis categories, only the general and testis categories show a statistically significantly fewer average number of interactions than the embryonic categories, when significant (Additional file 12).