Figure 2

Cells lacking Gα8, Gα9 or Gβ proliferate faster than wild-type cells. (A) Cells were diluted to 2 × 10⁵cells/ml in HL5 and the cell density was measured daily. The graph shows means ± s.e.m. from three independent experiments. The differences between the maximum cell density attained by wild-type cells and aprA ^-, cfaD ^-, gα8 ^-, gα9 ^-, and gβ ^- mutants are significant with P < 0.05, whereas the difference between the wild-type maximum density and the gα2 ^-, gα5 ^-, and gα7 ^- maximum densities are not significant (one-way ANOVA, Dunnett's test). (B) The data from the first five days was plotted using a log scale for the density. The absence of error bars indicates that the error was smaller than the plot symbol.